Suchergebnisse
25. Juni 2024 · The BCA method is widely used in protein isolation and purification due to its advantage (over the modified Lowry method and any Coomassie dye-based assay) of being compatible with samples containing up to 5% detergents.
Vor 4 Tagen · An improved assay, the Thermo Scientific Pierce BCA Protein Assay using bicinchoninic acid, is a chelator-based assay with exceptional linearity and low protein-to-protein variability that was quickly established as the “gold standard” for protein assays.
Vor 19 Stunden · Some of the most common methods employed for protein estimation include Bradford’s assay, Lowry’s assay, and the BCA assay. Activated furans have been reported to react with amine functionalities to afford highly coloured Donor Acceptor Stenhouse Adducts (DASAs).
Vor einem Tag · BCA assays or microBCA assays were performed according to the manufacturer's instructions. 2.12 Western blot analysis Equal amounts (or equal volumes of samples) of cellular or sEV proteins were separated using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes (GE Healthcare, Chicago, IL, USA).
Vor 6 Tagen · In this study, papaya PDENs were isolated by a PEG6000-based method and characterized by dynamic light scattering (DLS), transmission Electron Microscopy (TEM), bicinchoninic acid (BCA) assay method, GC-MS analysis, total phenolic content (TPC) analysis, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.
28. Juni 2024 · Liquid biopsy is a less invasive and more accurate alternative. Metabolomic analysis of extracellular vesicles (EVs) holds a promise for detecting non-genetic alterations and biomarkers in PCa diagnosis and risk as-sessment.
13. Juni 2024 · Mark Pawlicki, Senior Product Manager at MilliporeSigma, summarizes the challenges of protein quantitation and the opportunities provided by the well-known bicinchoninic acid (BCA) assay: “The complexity of quantifying protein in a sample is often taken for granted.
