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Vor 16 Stunden · Immunoprecipitation: The sheared chromatin is incubated with an antibody specific to the target protein. The magnetic bead coupled antibody pulls down the protein-DNA complexes. H3K4me3 and IgG antibodies are used as positive and negative controls, respectively.
Vor 16 Stunden · Chromatin immunoprecipitation (ChIP) is a procedure used to examine the targeting of a protein to specific regions of chromatin in vivo [].The procedure comprises a series of steps, including the crosslinking of the protein to chromatin in vivo, the fragmentation of chromosomal DNA to small sizes, and the immunoprecipitation of protein–chromosomal DNA complexes with an antibody against the ...
Vor einem Tag · Immunoprecipitation of AML1–ETO SP from mammalian cells. ... How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers. Nat. Struct. Mol. Biol. 14 ...
Vor 5 Tagen · Integration of the dATAC peak data with the chromatin immunoprecipitation sequencing data revealed that the Mediator complex and transcription factors bound most strongly to the bidirectionally transcribed dATAC peaks, followed by unidirectionally transcribed dATAC peaks, but very weakly to untranscribed dATAC peaks (Fig. 2H and fig. S12, B and C).
Vor einem Tag · Chromatin immunoprecipitation ChIP was performed as described. 95 Briefly, 1x10 7 cells per IP were crosslinked with 37% formaldehyde. Cells were lysed in 1 mL lysis buffer (150 mM NaCl, 1% NP-40, 0.5% deoxycholate, 0.1% SDS, 50 mM Tris, 5 mM EDTA, pH 8.0, and 1x protease inhibitor cocktail).
Vor 4 Tagen · Genome-wide AP2-LT binding data from chromatin immunoprecipitation followed by sequencing (ChIP-seq) experiments is located as fully processed on NCBI Gene Expression Omnibus (GEO) [GSE212052]. ChIP-seq genome tracks (.bigwig) and unprocessed gel images are attached to the supplement and located on the Zenodo database [10.5281/zenodo.7007554].
Vor 5 Tagen · The genomic binding sites of TFs are typically mapped using chromatin immunoprecipitation followed by sequencing (ChIP-Seq) (Johnson et al. 2007; Park 2009). In ChIP-Seq, TF binding sites are determined by sequencing DNA fragments that are obtained after purification of cross-linked protein-DNA complexes using a specific antibody to ...
